Chemical Cross-linking and Covalent Labeling: From Proteins to Cellular Networks

January 24 - 27, 2019  |  Hilton St. Petersburg Bayfront

Featuring keynote lectures by Ruedi Aebersold (ETH Zurich), Brian Chait (Rockefeller University), and Carol Robinson (Oxford University).

Thursday, January 24, 2019

6:00-7:00 pm, Registration

7:00-7:05 pm, Opening Remarks: Andrea Sinz, Richard Vachet & Lan Huang

7:05-8:05 pm, Keynote Lecture: "Alternative Ways to Study Protein Interactions"; Carol Robinson, University of Oxford

8:05-10:00 pm, Reception

Friday, January 25, 2019

7:00-8:30 am, Continental Breakfast

8:30-10:00 am, Developing Cross-linking Mass Spectrometry to Delineate Protein Structures
Each talk is 25' followed by several minutes for Q&A.

  • "Protein Structure Prediction by the Combined Approach of Short Distance Crosslinking and Discret Molecular Dynamics"; Christoph Borchers, University of Victoria, Canada
  • "Improving Mass Spectrometry Analysis of Protein Structures with Arginine-Selective Chemical Cross-linkers"; Meng-Qiu Dong, National Institute of Biological Sciences, China
  • "Cross-linking/Mass Spectrometry Workflows Based on MS-Cleavable Cross-Linkers and the MeroX Software for Studying Protein Structures and Protein-Protein Interactions"; Andrea Sinz, Martin Luther University Halle-Wittenberg

10:00-10:15 am, Coffee Break

10:15-11:45 am, Developing Cross-linking Mass Spectrometry to Delineate protein Structures
Each talk is 25' followed by several minutes for Q&A.

  • "Cross-linking Mass Spectrometry Strategies to define Interaction and structural dynamics of Protein Complexes"; Lan Huang, University of California, Irvine
  • "Kinetochore Assembly and Function: Insights by Chemical Crosslinking and Mass Spectrometry"; Franz Herzog, Ludwig-Maximilians-Universität München
  • "Compositional Dynamics of Protein Complexes"; Natalie Romanov, EMBL Heidelberg

11:45 am-12:00 pm, Group Photo

12:00-1:15 pm, Group Lunch, provided by ASMS

1:15-3:15 pm, Oxidative Labeling
Each talk is 25' followed by several minutes of Q&A.

  • "Oxidative Footprinting and Crosslinking for Determining Protein/Protein and Protein/Peptide Interfaces"; Michael Gross, Washington University
  • "Structure and Dynamics of Macromolecules Using Footprinting"; Mark Chance, Case Western Reserve University
  • "Reproducibility and Robustness in FPOP:  the Need for Community Standards for Radical Dosimetry"; Josh Sharp, University of Mississippi
  • "Functional Implications of Protein Oxidation: Turning Cytochrome C into an Apoptotic Peroxidase"; Lars Konermann, Western University, Canada

3:15-7:00 pm, Free Time

7:00-7:45 pm, Keynote Lecture: "Surveying and Mapping Macromolecular Cellular Terrains"; Brian Chait, Rockefeller University

7:45-8:00 pm, 5 x 3 minute Poster Highlight Talks

  • Heather O'Neill; “Aptamer-Based Affinity Labeling Identifies a Surface Spliceosomal Complex that When Internalized Induces Aberrant Splicing and Cell Death in B-Cell-Lymphoma Cells”.
  • Esben Trabjerg; “Feasibility of Succinimidyl-Based Crosslinking at Slightly Acidic Conditions”.
  • Sabine Wittig; “Cross-Linking Intact Synaptic Vesicles Reveals Protein Interactions Networks that Mediate Membrane Fusion in the Neuronal Synapse”.
  • Jayanta Kishor Chakrabarty; “High Confidence Identification of Protein Cross-Linking by Next Generation Dual Cleavable Cross-Linking Technology (DUCCT)”.
  • Bingqing Zhao; “Multiple Dissociation Methods in Conjunction with an ETD Cleavable Cross-Linker Facilitate the Identification of Cross-Linked Peptides”.

8:00-10:00 pm, Poster Session I

Saturday, January 26, 2019

7:00-8:30 am, Continental Breakfast

8:30-10:30 am, Software Development and Structural Modeling
Each talk is 25' followed by several minutes for Q&A.

  • "Incorporating XlinkX into Integrative Structural Biology"; Albert Heck, University of Utrecht
  • "The MaxQuant Software for Cross-Linking/Mass Spectrometry"; Jürgen Cox, MPI Martinsried
  • "Reliable Identification of Cross-Links with Non-Cleavable Linkers", Robert Chalkley, University of California, San Francisco
  • "Macromolecular Structure and Dymamics Based on Cross-Links"; Dina Schneidman, Hebrew University

10:30-10:45 am, Coffee Break

10:45-11:45 am, 4 x 15 minute Short Poster Talks

  • Mark Larance; “Global Liver Protein Crosslinking Reveals a Direct Interaction between the RISC complex and the Ribosome”.
  • Rosa Viner; “Protein Complex Dynamics Using Quantitative Cross-linking Mass Spectrometry”.
  • Saiful Chowdhury; “Toll-Like Receptor 2 Interactome by Mass Spectrometry-Based Co-Immunoprecipitation (Co-IP) Crosslinking Proteomics”.
  • Julian Mintseris; “High-Density Chemical Cross-Linking for Modeling Protein Interactions”.

11:45-1:00 pm, Lunch on your own

1:00-2:30 pm, in vivo Labeling and Analysis of Cellular Networks
Each talk is 25' followed by several minutes for Q&A.

  • "Quantitative Interactome Dynamics: A Look under the Hood"; Jim Bruce, University of Washington, Seattle
  • "In-Cell Footprinting for Proteome-Wide Structural Biology"; Lisa Jones, University of Maryland
  • "Proximity Proteomics: from Organelles to Complexes"; Anne-Claude Gingras, Lunenfeld-Tanenbaum Research Institute, Canada

2:30-3:00 pm, 2 x 15 minute Short Poster Talks

  • Casimir Bamberger; “Covalent Protein Painting Reveals Aberrant Protein Folding in Cells and Tissues in vivo”.
  • Haiyuan Yu; “MaXLinker: an Innovative “MS3-Centric” Proteome-Wide Cross-Link Search Engine with High Specificity and Sensitivity”.

3:00-7:00 pm, Free Time

7:00-7:30 pm, 2 x 15 minute Short Poster Talks

  • Ryan Bomgarden; “Right Tools for the Job: New compounds and Sample Preparation Reagents for Studying Chemically Cross-Linked Proteins”.
  • Gianluca Degliesposti; “Cross-Linking and Mass Spectrometry for the Structural and Functional Investigation of the 3’-End mRNA Cleavage and Polyadenylation Factor (CPF)”.

7:30-8:00 pm,7 x 3 minute Poster Highlight Talks

  • Therese Dau; “Sequential Digestion with Trypsin and Elastase Improves Sequence Coverage in Cross-Linking/Mass Spectrometry”.
  • Christian Tüting; “Structural Insight into the Polyadenylation Complex”.
  • Laurence Angel; “Weak Acid-Base Interactions of Histidine and Cysteine Affect the Charge States, Tertiary Structure, and Zn(II) Labeling of HeptaPeptides”.
  • Mowei Zhou; “Pseudo-enzyme PDX1.2 and its Interaction with Active Analogs Explored with Native Mass Spectrometry”.
  • Marshall W. Bern; “How the Flu Takes Off Its Coat:  Cross-Linking Study of pH-Induced Changes of the Influenza A Virus Matrix Layer”.
  • Ann English; “Profiling Oxidative Proteoforms to Discover New Redox Functions of Proteins in Cells: the Case of Cytochrome C Peroxidase”.
  • Oleg Klykov; in-situ Crosslinking Mass Spectrometry Provides a High-Resolution Structural Model of Fibrin Clots”.

8:00-10:00 pm, Poster Session II

Sunday, January 27, 2019

7:00-8:30 am, Continental Breakfast

8:30-10:00 am, Defining Protein Aggregates/Complexes
Each talk is 25' followed by several minutes for Q&A.

  • "Insight into beta-2-Microglobulin Amyloid Formation and Inhibition Using Covalent Labeling Mass Spectrometry"; Richard Vachet, University of Massachusetts
  • "Diazirine-Based Reagents for Structural Mass Spectrometry of Complex Systems"; David Schriemer, University of Calgary, Canada
  • "Methods and Applications for the Thermodynamic Analysis of Protein-Ligand Complexes on the Proteomic Scale"; Michael Fitzgerald, Duke University

10:00-10:15 am, Coffee Break

10:15-11:15 am, Defining Protein Aggregates/Complexes (Continued)
Each talk is 25' followed by several minutes for Q&A.

  • "Structural Basis of Cullin-RING E3 Ligase Regulation by the COP9 Signalosome Using Integrative Mass Spectrometry"; Argyris Politis, King's College London
  • "Integrating Surface-Induced Dissociation into a Native MS Workflow"; Vicki Wysocki, Ohio State University

11:15 am -12:15 pm, Keynote Lecture: "Towards the Modular Proteotype", Ruedi Aebersold, ETH

12:15-12:30 pm, Closing Remarks