Conferences

Preliminary Program

Opening Lecture

  • Patrick Limbach (Brown University), Decoding the Chemical Language of RNA by Mass Spectrometry

Sample Preparation for Nucleic Acid Assays by LC-MS

  • Eric Wolf (NEB), Sequence-Specific RNases for Bottom-Up RNA LC-MS/MS: New Tools and Workflows
  • Bala Addepalli (Waters), RNase MC1 and Cusativin: pH-Modulated Cleavages for end-to-end analysis of RNA Therapeutics
  • Afrand Kamali (Novo Nordisk), Current and Emerging Extraction Methods for LC-MS Bioanalysis of Oligonucleotide Therapeutics

Strategies for the Optimization of the Chromatographic Separation of Nucleic Acids

  • Michael Bartlett (University of Georgia), Fundamentals of Ion-Pair Reversed Phase Liquid Chromatography Mass Spectrometry
  • Todd Maloney (Eli Lilly), Resolving Sequence and Stereochemical Impurities in Therapeutic Oligonucleotides via Multi-Mode Separations
  • Martin Gilar (Waters), Advances in LC and MS Analysis of Oligonucleotides: Minimizing Non-Specific Adsorption

Data Processing and Interpretation for LC-MS Data

  • Sam Wein (University of Tuebingen), Scaling RNA Mass Spectrometry: Robust Identification and Label-Free Quantification with NucleicAcidSearchEngine
  • James Williamson (Scripps), Finding and Quantifying RNA Modifications using Pytheas
  • Patrick Limbach (Brown University), RAMM 2.0

Overview of Current Vendor Solutions for LC-MS Software Solutions for Nucleic Acid Analysis

  • Steven Broome (Protein Metrics), Oligonucleotide Characterization Is More than Just the Sum of the Parts
  • Thomas Walker (Agilent), An Integrated Workflow for the Intact Characterization and Sequencing of Oligonucleotides and Their Impurities by High-Resolution LC-MS
  • Martin Gilar (Waters), LC/MS RNA Mapping with CONFIRM Sequence – A Waters Connect Application
  • Keeley Murphy (Thermo Fisher Scientific), BioPharma Finder for Nucleotide and mRMA Analysis
  • William McIntyre  (Novatia), Streamlining Oligonucleotide MS/MS Sequencing with ProMass 6 HR: High-Confidence Data Interpretation
  • Michael Freitas (Mass Matrix), mmBiologics — From Intact Mass to Verified Sequence: Automating Nucleic Acid Fragment Interpretation

Identification by Fragmentation with Mass Spectrometry

  • Dan Fabris (University of Connecticut), Capillary Electrophoresis Mass Spectrometry for the Analysis of Oligonucleotide Mixtures
  • Ben Garcia (Washington University), LC-MS/MS for the Quantitative Analysis of Nucleosides and Oligonucleotides
  • Robert Ross (Thermo Fisher Scientific), Linear Ion Trap fragmentation of RNA

Strategies for Optimizing LCMS Analysis in Early Drug Development for Nucleic Acid Therapeutics

  • Xien Yu Chua (Alnylam Pharmaceuticals), Characterizing Single-Stranded Lipophilic siRNA Metabolites in Plasma 
  • Dan Fabris (University of Connecticut), Capillary Electrophoresis Mass Spectrometry for the Analysis of Oligonucleotide Mixtures
  • Tao Wei (GondolaBio), Characterization Strategies for Phosphorodiamidate Morpholino Oligomers Using Multidimensional LC and MS/MS

Quality Control Strategies

  • Kui Yang (FDA), Oligonucleotide Active Ingredient Sameness and Impurity Comparability Studies by Mass Spectrometry
  • Camila Ortega (Abzena), Leveraging Sample Preparation and Treatment Strategies to Enable Robust LC‑MS Analysis of Nucleic Acids
  • Mark Hail (Novatia), LC-MS Methods and Automated Data Processing for Ensuring Oligonucleotide Quality

Large RNA Modalities

  • Weijing Liu (Thermo Fisher Scientific), Advancing Large RNA Analysis Through Charge Detection Mass Spectrometry
  • Camila Ortega (Abzena), Understanding Large RNA CQAs to Guide Development of Fit‑for‑Purpose Analytical Methods
  • Michael Sutton (Eli Lilly), Characterization Method Development for sqRNA

Schedule

Wednesday, November 11

Arrivals for out-of-town attendees

Thursday, November 12

7:30 - 8:30 am, Badge Pick-up

8:00 am - 5:00 pm, Workshop Program

5:00 - 6:00 pm, Happy Hour

Friday, November 13

8:30 am - 5:00 pm, Workshop Program

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