November 2-3, 2017
Hyatt Regency Boston Harbor
Ying Ge, University of Wisconsin-Madison
Ljiljana Paša-Tolić, Pacific Northwest National Laboratory
Top-down mass spectrometry (MS)-based proteomics is arguably a disruptive technology for the comprehensive characterization of proteoforms that arise from genetic variations, alternative splicing, and post-translational modifications (PTMs). In the top-down approach, intact proteins are analyzed without proteolytic digestion. Subsequently, specific proteins of interest can be isolated and modification sites can be mapped by tandem MS (MS/MS) strategies. Thus, it provides a “bird’s eye” view of all proteoforms to decipher the PTM codes together with genetic variations that regulate cellular signaling in healthy and diseased states. Over the last decade, significant strides have been made to advance top-down MS and bring it to the mainstream. As a result, interest in top-down MS have grown considerably and a number of studies have already showcased the potential of top-down proteomics for the unraveling of disease mechanisms and discovery of new biomarkers.
In this workshop, world-leading experts in top-down MS-based proteomics will present the most advanced technologies and approaches. The workshop will cover a wide range of topics, including sample preparation, intact protein separation, MS and MS/MS for intact proteins, bioinformatics and data analysis tools for top-down proteomics, as well as the biomedical applications. Each presentation will be a tutorial aimed at beginners/newcomers to the top-down proteomics field. The Workshop will conclude with a dynamic moderated discussion/quiz session.